GENERATION, CHARACTERIZATION AND PHARMACOKINETIC STUDY OF OFLOXACIN-LOADED CASTOR OIL BASED NANOEMULSION
Journal: Acta Chemica Malaysia (ACMY)
Author: Abayomi Bamisaye, Catherine Oluyemisi Eromosele, Enock Olugbenga Dare, Sivakumar Manickam, Oluseyi Adeboye Akinloye, Mopelola A. Idowu, Ige A. Raphael
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The challenges associated with effective drug delivery necessitates the development and adoption of modern approach. This research aims to improve the bio-potency of ofloxacin (OF) against drug resistant strains of microorganism using nanoemulsion. The emulsification was achieved by mechanochemical process. The morphological characterization of emulsion was carried out using Cryogenic-TEM (Cryo-TEM). The electro-kinetic properties and emulsion size was done using Zetasizer and Photon Correlation Photo-spectrometer (PCS). Drug-emulsion interaction study using FTIR. The antimicrobial studies of Ofloxacin (OF), non-drug loaded emulsion (COAB), drug-loaded emulsion (COAB+OF) was carried out on P. aeruginosa and pharmacokinetics were established using HPLC. Cryo-TEM micrograph showed spherical morphology with mean particle size of 166.8 and 177.5 nm for COAB and COAB+OF respectively. While PCS and Zetasizer recorded a mean value of 0.156 and -26.1 mV for COAB and 0.341, and -2.72 mV for COAB+OF respectively. This shows that the colloids are nano-sized, charged and metastable. FTIR results shows O-H absorption at 3570 – 3200 cm-1, likewise CH3 and CH2 at 3050-2895 cm-1 for OF, COAB and COAB+OF respectively. This suggests encapsulation and wholeness with no drug-excipient interactions. The antimicrobial study shows COAB+OF to me more bio-potent that OF with zone of inhibition value of 12.8±0.3 mm compared to 8.0±0.2 mm in OF at 62.50 mg/ml. HPLC showed a maximum OF concentration (Cmax) of 2.27 μg/mL at 150 min which is the drug release maxima. This study showed that encapsulating ofloxacin in castor oil based nanoemulsion system improves its bio-potency.